Transcriptome-wide Analysis of Intracranial Artery in Patients with Moyamoya Disease Showing Up-regulation of Immune Response, and Down-regulation of Oxidative Phosphorylation and DNA Repair

Micro-samples of the middle cerebral artery (MCA) were collected from patients with MMD (n=11) and those with control (n=9). Using microarray techniques, transcriptome-wide analysis was performed. Comparison of the MCA gene expression between patients with MMD and control detected 62 and 26 genes whose expression was significantly (P<0.001, fold change>2) up- or down-regulated in the MCA of MMD. Gene set enrichment analysis of genes expressed in the MCA of MMD revealed positive correlations with genes involved in antigen processing and presentation, dendric cells pathway, cytokine pathway, and interleukin 12 pathway, whereas negative correlations with genes involved in oxidative phosphorylation and DNA repair. In addition, quantitative polymerase chain reaction analysis confirmed decreased gene expressions of tetraspanin 2 and ras homolog family member Q in MCA of MMD (P<0.05). Micro-samples of the middle cerebral artery (MCA) were collected from patients with MMD (n=11) and those with control (n=9). GSE157628 includes re-used Samples from GSE141022. The GSE157628 Samples were re-used in GSE189993.