Gene Sequences of Endophytic Bacteria in Capsicum annuum L.

This study investigated the endophytic microbial communities in different ecological niches of pepper seedlings at various stages following Ralstonia solanacearum infection, combined with the pathogen invasion process, to preliminarily explore the dynamic shifts of endophytes triggered by R. solanacearum infection.The copy number of R. solanacearum was quantified using quantitative fluorescence PCR (qPCR). Samples from roots, stems, and leaves of both infected and healthy pepper plants were collected at 1, 4, and 7 days post-inoculation (dpi). High-throughput amplicon sequencing was employed to analyze the endophytic bacterial and fungal communities. Dual-dimensional analyses integrating microbial copy numbers and community structures were conducted to elucidate the spatiotemporal dynamics of endophytic microbiota and pathogen proliferation characteristics under R. solanacearum invasion. .