Caenorhabditis elegans infected with Enterococcus

Young adult N2 Caenorhabditis elegans were infected with Enterococcus faecalis or Enterococcus faecium for 8 h to determine the transcriptional host response to each enterococcal species. Analysis of differential gene expression in C. elegans young adults exposed to four different bacteria: heat-killed Escherichia coli strain OP50 (control), wild-type E. faecalis MMH594, wild-type E. faecium E007, or Bacillus subtilis PY79 (sigF::kan). Samples were analyzed at 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Brain-heart infusion agar plates (10 ug/ml kanamycin) were used. There are 13 samples total: 3 biological replicates of E. faecalis-infected animals, 3 biological replicates of E. faecium-infected animals, 4 biological replicates of heat-killed E. coli-fed controls, and 3 biological replicates of B. subtilis-fed controls. All treatments were performed in parallel except for two heat-killed E. coli-fed controls, Jos5531(OP504) and Jos5532(OP505), which were processed together on a different day from the other samples.