Nanopore long-read RNA sequencing in untreated (UN), sodium arsenite (SA) and heat shock (HS) stressed HeLa cells

Recent completion of the telomere-to-telomere (T2T) genome assembly has enabled a comprehensive characterization of pericentromeric Sat?, SatII, Sat? and centromeric a-satellite repeats. Sat? DNA constitutes ~1.56% of the genome with a reported localization across 16 chromosomes. The transcription activity of Sat? DNA across genome and the sequence of Sat? transcripts remained largely unclear. We performed nanopore long-read RNA sequencing (RNA-seq) in untreated (UN), sodium arsenite (SA: 0.1mM, 5 h) and heat shock (HS: 42°C, 2 h; 37°C, 1 h) stressed HeLa cells to characterize Sat? transcripts . Since a portion of Sat? transcripts is non-polyadenylated, We performed polyadenylated (poly(A)+) and rRNA-depleted (ribo-) nanopore cDNA long-read RNA-seq. Overall design: We performed polyadenylated (poly(A)+) and rRNA-depleted (ribo-) nanopore long-read RNA-seq in untreated (UN), sodium arsenite (SA) and heat shock (HS) stressed HeLa cells. Two biological independent experiments in each condition were included.