Primary data associated with the manuscript entitled "Nicotinamide Riboside Augments Human Macrophage Migration <i>via</i> SIRT3 Mediated Prostaglandin E2 Signaling".

NAD⁺-boosting <i>via</i> nicotinamide riboside (NR) confers anti-inflammatory effects. However, underlying mechanisms and therapeutic potential remain incompletely defined. Here we showed NR increased the expression of CC-chemokine receptor 7 (CCR7) in human M1 macrophage by flow cytometric analysis of cell surface receptors. Consequently, chemokine ligand 19 (CCL19, ligand for CCR7) - induced macrophage migration was enhanced following NR administration. Metabolomics analysis revealed Prostaglandin E2 (PGE2) was increased by NR in human monocytes and in human serum following <i>in vivo</i> NR supplementation. Furthermore, NR-mediated upregulation of macrophage migration through CCL19-CCR7 was dependent on PGE2 synthesis. We also demonstrated that NR upregulates PGE2 synthesis through SIRT3-dependent post-transcriptional regulation of Cyclooxygenase 2 (COX-2). The NR-SIRT3-Migration axis was further validated by the scratch-test model where NR and Sirt3 promoted more robust migration across a uniformly disrupted macrophage monolayer. Thus, NR-mediated metabolic regulation on macrophage migration and wound healing may have therapeutic potential for the topical management of chronic wound healing.