Torsten Wittmann, Gary M. Bokoch, Clare M. Waterman-Storer (2011) CIL:11844, Potorous tridactylus, epithelial cell. CIL. Dataset

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell. Fast retrograde flow occurs in the lamellipodium and slow retrograde flow in the lamellum. The cell was microinjected with X-rhodamine-conjugated actin and observed by spinning disk confocal microscopy. Fluorescent speckle time-lapse series were acquired on a spinning disk confocal microscope with a 100/1.4 NA Plan Apo objective lens. Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 5B of associated manuscript (J Cell Biol, 161:845-851, 2003).

在表达Rac1(Q61L)的PtK1细胞中，肌动蛋白的动态变化。在伪足区域出现快速逆向流动，而在层状区域出现缓慢逆向流动。细胞通过显微注射引入与X-罗丹明偶联的肌动蛋白，并采用旋转盘共聚焦显微镜进行观察。在配备100/1.4 NA Plan Apo物镜的旋转盘共聚焦显微镜上采集了荧光斑点的时间序列图像。图像每隔5秒拍摄一次，并以15帧/秒的速度回放。静态图像可参见相关文献的图5B（J Cell Biol, 161:845-851, 2003）。