ERK inhibitors display cell type-specific relation between ERK activity and cell viability

RNA was isolated for 9 cell lines using TRIzol (ThermoFisher) and RNA Clean & Concentrator kit (Zymo Research), and then subjected to DNAase I treatment (ThermoFisher). In total, 9 libraries were prepared from isolated total RNA using TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) based on poly(A) enrichment protocol (TruSeq Stranded mRNA Reference Guide, Illumina). RNA samples having RIN (RNA Integrity Number) > 8.0 were used for library preparation. The final cDNA library sizes were approximately 260-270 bp. cDNA libraries were equally pooled and sequenced on a NextSeq 550 System (Illumina) to generate 75 nucleotide single-end reads.