Characterization of RNA binding proteins by in situ serial crystallography at room temperature using ChipX

Our objective is to test of an optimized version ChipX, a chip designed for crystal production and in situ serial characterization, before its dissemination in the crystal
growth community. We will apply the ChipX technology to the study of a series of RNA binding proteins that are of direct biological interest for our team. They
includes bacterial aminoacyl-tRNA synthetases and tRNA CCA-adding enzymes, Arabidopsis NFD2 factor. ChipX will be used to optimize crystallization
conditions, as well as soaking with ligands and inhibitors, then for in situ data collection on FIP2.