StYLS draft genome, predicted proteome & Assembly of insect SRA accessions - part 2

To identify insect species potentially associated with Ophiocordyceps-related yeast-like symbionts, transcriptomic SRA reads were aligned against the StYLS coding sequences of 18 ribosomal proteins and cytochrome c oxidase subunit I (COI). Read mapping was performed with Magic‑BLAST v1.7.0.

Libraries with ≥20 aligned reads were downloaded. Reads were then subjected to quality control using the BBTools suite and assembled with SPAdes v4.2.0 in “meta” mode using default parameters. The resulting transcripts were queried against the NCBI core-nr database using DIAMOND BLASTx to identify fungal transcripts belonging to genera within the family Ophiocordycipitaceae.

The second part (43 files) of the 143 FASTA files generated from the assembled libraries are provided (please see 10.5281/zenodo.18506981 for the first 100 files).

Filenames contain the insect species name and the NCBI accession number of the analyzed library.

The draft genome and the predicted proteome of the Scaphoideus titanus yeast-like symbiont (StYLS), generated using the MAKER annotation pipeline (v. 2.31.11) and Funannotate (v. 1.8.17), are also provided.