s-Adenosylmethionine (SAM) diet defines offspring epigenetic modification and intergenerational immune response.

At the beginning of the experiment, a concentration of 50µM was used The treatment of Caenorhabditis elegans with MS adenosylmethionine has been found to promote the natural immunity of nematodes and increase their survival rate in Pseudomonas aeruginosa. We found that this elevated immunity has an epigenetic phenomenon that can be inherited to its offspring. To further explore its molecular mechanism, we aim to investigate the gene expression differences between S-adenosylmethionine treatment and untreated, as well as the similarities and differences in gene expression between S-adenosylmethionine treatment and offspring Overall design: Using a concentration of 50 µ M-adenosylmethionine was used to treat Caenorhabditis elegans, and the nematode was placed in Pseudomonas aeruginosa to test its natural immune ability.