Identification and characterization of miRNAs in temperature-dependent sex determination (TSD) of Chinese pond turtles (Mauremys reevesii)

In the present study, the eggs of Chinese pond turtles (Mauremys reevesii) were incubated at three temperatures (26?, 29? and 32?). During the thermosensitive period (TSP) of incubation, the adrenal-kidney-gonad (AKG) complexes were sampled, and a comprehensive investigation for miRNAs was performed using next-generation small RNA sequencing. Overall design: Global miRNA expression kinetics during sex determination in a TSD species have been extensively profiled.