RNA-seq of prostate cancer cell lines co-culture with osteoblasts or osteoclasts

Clinically, osteolytic phenotype is rare in prostate cancer. The molecular mechanism of bone metastasis in PCa is not fully understood. We performed RNA-seq to identify osteogenic and tumor associated roles in prostate cancer by a co-culture of osteoblasts (MG63) and prostate cancer cells (C4-2, C4-2B, 22Rv1 and DU145). We compared osteoblastic prostate cancer with osteolytic prostate cancer to evaluate the difference in phenotype of bone metastasis. Overall design: Osteolytic prostate cancer (DU145 and 22RV1) and osteoblastic prostate cancer (C4-2 and C4-2B) were either mono-cultured or co-cultured with MG63 in horizontal co-culture system (NICO-1). Total RNA was extracted after 5days culture.