"BlinkFusion - Filaments and STORM validation"

"This DataPort submission contains two datasets used for quantitative analysis of filament labeling and single-molecule photophysics in fluorescence microscopy using the BlinkFusion platform.The first dataset consists of filament data derived from confocal microscopy of nanobody-labeled tubulin using aC-dots. The dataset is organized as folders containing representative images of the top-ranked regions of interest (ROIs) selected during preprocessing, along with the corresponding batch-processing results from Stretching Open Active Contours (SOAC) filament analysis. These data include filament-level metrics such as length, intensity, contrast, and continuity, and are directly compatible with the filament analysis workflow implemented in BlinkFusion.The second dataset consists of STORM microscopy image sequences acquired from two samples of Cdots imaged in photoswitching buffer. The dataset includes time-series TIFF files from STORM acquisitions along with the results from ThunderSTORM localization processing (following our own analysis hrough our ThunderSTORM guide), this enable extraction of single-molecule localization and blinking statistics, including duty cycle, survival fraction, photon yield, and switching cycles.Together, these datasets were used as validation experimental data for the BlinkFusion software"