Gene expression data from MGH-U3 following FGFR3 knock-down using FGFR3 siRNAs

To better understand the molecular mechanisms underlying altered-FGFR3 oncogenic activity in bladder carcinomas, we made use of MGH-U3 cell lines, which were derived from a human bladder tumor and endogenously expressed a mutated activated form of FGFR3 (FGFR3-Y375C), the growth and transformation of these cell lines being dependent on activated-FGFR3 activity. We conducted a gene expression analysis using Affymetrix DNA arrays in this cell line treated or not with FGFR3 siRNAs. MGH-U3 cells were transfected or not with siRNA FGFR3. mRNA was extracted after 72h and gene expression analysis was assessed using the Human Exon 1.0 ST (Affymetrix) array