Effects of lysine and methionine on mRNA expression of candidate transcription factors by primary bovine mammary epithelial cells

The dataset originates from an experiment investigating the effects of lysine and methionine on mRNA expression of candidate transcription factors in primary bovine mammary epithelial cells. The objective of this study is evaluate if and when the candidate gene expression in bovine mammary epithelial cell is affected after imposing an essential amino acid deficiency in vitro, and to evaluate effects of essential amino acid deficiency on protein synthesis, endoplasmic reticulum size, cell proliferation and lipogenesis.