Single-nuclei sequencing of the mouse hippocampus and caudate putamen at young and old age

Aging is a key driver of cognitive decline and the predominant risk factor for several neurodegenerative diseases. Recent behavioral studies as well as structural and functional MRI data suggest that aging does not impact the brain in a uniform manner but follows region- and age-specific trajectories. Yet so far, quantitative analyses of the molecular dynamics in the aging brain have been limited to few regions at low temporal resolution. Here we performed single-nuclei sequencing (Nuc-seq) of hippocampus and caudate putamen isolated from young (3 months) and old (21 months) old mice. Overall design: Single-nuclei RNA transcriptomes of hippocampus and caudate putamen obtained from brains of young and old mice using 10x Genomics Drop-seq (v3.1).