Transcriptomes From Hepatocellular Carcinoma and Normal Liver Tissues

This dataset describes a specific study group. Nine patients were included in the study group, all with clinical diagnosis of hepatocellular carcinoma (HCC). The study group consisted of seven male patients and two female patients of age from 45 to 80. Patients had a single liver tumor and indications for surgery were set according to the Barcelona Clinic Liver Cancer (BCLC) staging system. All patients were evaluated according to BCLC criteria and belong to 0-A stage. The diagnosis of HCC before the operation was confirmed by radiology examinations and/or histopathological findings. None of the patients received any other specific treatment before the surgery. HCC patients underwent radical liver surgery, and according to subsequent histology results – three patients were Stage I, two patients Stage II, three Stage III and one Stage IV HCC. During operation, no dissemination of tumor was found, and all resected specimens were with R0 resection margins. RNA isolation from tissues and transcriptome sequencing and primary data analysis was performed by Personal Genomics (Italy). After RNA quality control, mRNA-seq library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) (https://www.neb.com/en/products/e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina) and libraries were sequenced on an Illumina NovaSeq 6000 (https://www.illumina.com/systems/sequencing-platforms/novaseq.html) using 150 nt paired-end reads. The mRNAsequencing generated an average of 72 Million paired-end fragments/sample. The average alignment rate was of 97.80%.