RNA sequencing of E. coli NCM3722 over the course of glucose to acetate growth transition

To understand the gene response during the glucose to acetate diauxic transition, we grew E. coli in minimal media with acetate and a small amount of glucose. Cells were collected and RNA was purified at different time points during the growth transition, including pre-shift (growth on glucose), 5 minutes, 15 minutes, 60 minutes and 120 minutes after glucose run-out, and steady state growth on acetate (post-shift). Overall design: Time-course monitoring of E. coli transcriptome by performing 6 RNA-sequencing experiments during glucose to acetate growth transition.