Introduced and indigenous viruses used: culture hosts for each isolate and antibodies for detection.
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Virus specific polyclonal antisera always used in ELISA tests on samples, except for generic potyvirus monoclonal antibodies from Loewe (inoculations to native species, and 2001+2009 field samples), tospovirus serogroups I, II and III from Sanofi (2001+2009 field samples), and luteovirus (1991 field samples) from DSMZ. In addition, polyclonal antiserum to Impatiens necrotic spot virus (INSV) from DSMZ was used once in 2009.AThese virus source plants were growing outside at DAFWA, South Perth either in hedges (PWV and HarMV-SP1) or as a potted plant (HarMV-Cgt), or in the glasshouse from infected seedlings grown from an infected seed stock (AMV-EW).BThese polyclonal antibodies had broad specificity, also detecting related viruses, BCMV = Bean common mosaic virus, BWYV = Beet western yellows virus.
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2015-12-02



