Gene deletion in actinomycetes using I-SceI meganuclease
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This protocol explains how to make deletions in actinomycete species using an efficient method to generate gene or gene cluster deletions by homologous recombination. This method does not introduce any other changes to the targeted organism’s genome, making sequential deletions easy to carry out. This is a two-step protocol.
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2.0 Streptomyces创建时间：
2014-11-25
