Mating response of three yeast species (S. cerevisiae, S. paradoxus and S. mikatae). Mating response of three yeast species (S. cerevisiae, S. paradoxus and S. mikatae)

We examined the mating response of three yeast species, by subjecting a-type cells from each of them to alpha-factor (6uM). The expression of cells before and after (60min) addition of alpha-factor were compared using S. cerevisiae cDNA microarrays. In addition, we verified our results with a true mating experiment: the expression of a-type and alpha-type cells from S. cerevisiae were compared before and after mixing them for 90 minutes (i.e. allowing them to mate). Keywords: yeast mating response, comparison among species Overall design: Three (or four) samples were processed in parallel for each species (biological replicates). All samples were measured by hybridization to S. cerevisiae cDNA microarray