The TFIIH kinase CDK7 governs RNA polymerase II initiation, elongation, termination, and RNA processing. The TFIIH kinase CDK7 governs RNA polymerase II initiation, elongation, termination, and RNA processing

In this study we perform precision nuclear run-on sequencing (PRO-seq) to measure the altered transcriptional response upon CDK7 inhibition or RNA-seq to elucidate the response of CDK7 inhibition on steady-state RNA. We performed a heat shock time course in PRO-seq with and without the Syros CDK7 inhibitor SY5609. Matching this data we performed three deeply sequenced RNA-seq experiments with SY5609 that include no heat shock, heat shock with recovery and long heat shock with no recovery. We additionally generated two more RNA-seq datasets in heat shock conditions with another Syros inhibitor, SY5102 and in a CDK7-analog sensitive OV90 cell line treated with 3MBPP1. Overall design: Examination of the altered transcriptional profile and steady-state RNA response with and without selective CDK7 inhibition upon heat shock stimulation.