RNA-seq to investigate the effect of curcumin on hypoxia-induced proliferation of human retinal pigment epithelium ARPE-19 cells

ARPE-19 RPE cells obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) were cultured in DMEM medium with 10% FBS (Thermo Fisher Scientific, Waltham, USA) at the incubator with 37 °C, 5% CO2 and 100% humidity. Final concentration of 15 µM curcumin (C7727, Sigma-Aldrich, St. Louis, USA) and 100 µM CoCl4 (10007216, Sinopharm Chemical Reagent, Shanghai, China) were added within serum-free medium for 24 h and 4 h respectively. RNA-seq was performed to investigate the transcriptome alteration.