Deciphering m6A dynamics at single-base level during planarian anterior-posterior (AP) axis specification. Deciphering m6A dynamics at single-base level during planarian anterior-posterior (AP) axis specification

Background: The establishment of the anterior-posterior (A-P) axis is a crucial step during tissue repair and regeneration. Despite the association reported recently of N6-methyladenosine (m6A) with regeneration, the mechanism underlying the regulation of m6A in A-P axis specification during regeneration remains unknown. Herein, we deciphered the m6A landscape at a single-base resolution at multiple time points during A-P axis regeneration and constructed the de novo transcriptome assembly of the Dugesia japonica planarian. Overall design: 18 samples from Header, Trunk and Tail were selected and performed RNA-seq and m6A-REF-seq.