Transcriptomic study of PMA-differentiated THP-1 cells following exposure to zinc oxide nanoparticles NM110

To identify differentially expressed genes and key biological pathways that define toxicity following nanoparticles exposure, we performed microarray analyses on PMA-differentiated THP-1 macrophages exposed for 4 h to 8 µg/mL of NP ZnO (50 % cell viability) and to 2 µg/mL of NP ZnO (50 % cell viability divided by 4 ). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 686098 Changes in gene expression in PMA-differentiated THP-1 cells incubated without (control) or with (2 or 8 µg/mL) of NP ZnO for 4 h were measured. For each condition, four biological replicates were performed : C1; C2; C3; C4 as controls, Z(2)1; Z(2)2; Z(2)3; Z(2)4, Z(8)1; Z(8)2; Z(8)3; Z(8)4 as exposed cells to 2 or 8 µg/mL respectively.