Role of IgD in B cell anergy. Mus musculus

To define the global landscape of mRNA changes in anergic B cells, and how this affected by surface IgD, mature CD93-CD23+ HEL-binding B cells were sorted from spleens of independent transgenic mice of the following genotypes: MD4, naïve B cells coexpressing IgM and IgD; MD4:ML5, anergic B cells coexpressing IgM and IgD; MM4, naive B cells expressing only IgM; MM4:ML5, anergic B cells expressing only IgM; DD6, naive B cells expressing only IgD; DD6:ML5, anergic B cells expressing only IgD. Overall design: Spleen cells from independent mice of each genotype were sorted on each of three sorting days (sort 1, 2, 3) and frozen. RNA was isolated from all frozen samples as a single batch and labelled and hybridized as a single experiment.