Biological characteristics of gene expression features in pancreatic cancer cell induced by proton and x-ray irradiation II

The aim of the study is to disclose the distinct alteration of biological characteristics of gene expression features in pancreas cancer cell, MIAPaCa-2, irradiated by proton and x-ray. The survived MIAPaCa-2 cells after irradiation with proton and x-ray were isolated and gene expression profiles were analyzed. MIAPaCa-2 cells were irradiated with proton or X-ray with 8 Gy dose in vitro. After 7 to 17 days, the colonies with survival cells were picked up and expanded. RNA was isolated from each expanded surviving cells (proton-irradiated surviving cells: n =5, X-ray-irradiated surviving cells: n=4) , followed by gene expression analysis.