EZH2 occupancy profiling by high throughput sequencing from macrophages non-treated (NT) or stimulated with LPS

To identify the potential genes that regulated by Ezh2, we immunoprecipitated chromatin from macrophages stimulated with or without LPS, and analyzed the precipitated DNA with deep sequencing (CHIP-Seq). Overall design: To examine EZH2 modification in macrophages,the chromatin of macrophages non-treated (NT) or stimulated for 2 h with LPS was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against EZH2 ( Active Motif 39901).