Data underlying the publication: Broad and specific anti-defense genes in bacteriophage genomes

This dataset supports the study <em>“Broad and specific anti-defense genes in bacteriophage genomes”</em> and contains experimental and computational data used to investigate how phages overcome bacterial immune systems. The research objective was to identify and functionally characterize phage-encoded genes that either broadly inhibit multiple bacterial defense pathways or specifically target individual systems. Data were collected through comparative genomics of diverse phage genomes, systematic genetic screens, molecular cloning, and infection assays measuring bacterial survival and phage propagation. The dataset includes the phylogenetic tree of all analysed phages, the protein alignment and HMM profiles of the identified anti-genes and the corresponding code that was used.

本数据集为本研究《噬菌体基因组中的广谱与特异性抗防御基因》提供数据支撑，收录了用于探究噬菌体对抗细菌免疫系统机制的实验与计算数据。本研究的核心目标为鉴定并功能表征噬菌体编码的两类抗防御基因：一类可广谱抑制多种细菌防御通路，另一类可特异性靶向单一细菌防御系统。本次研究的数据通过以下方式采集：对多样化噬菌体基因组开展比较基因组学分析、系统性遗传筛选、分子克隆，以及检测细菌存活率与噬菌体增殖情况的感染实验。本数据集涵盖所有分析噬菌体的系统发育树、已鉴定抗防御基因的蛋白质序列比对结果与隐马尔可夫模型（Hidden Markov Model，HMM）谱，以及研究所用的配套代码。