Exosomes from cyclic mice modulate liver transcriptome in estroupause mice independent of age

The aim of this study was to characterize the miRNA content and role of exosomes from cyclic mice in the modulation of liver transcriptome in estropausal mice. Two-month-old female mice were induced to estropause using 4-vinylcyclohexene diepoxide (VCD). At six months of age VCD-treated mice were divided in control group (VCD) and exosome group (EXO), with the latter receiving 10 injections of exosomes at 3-day intervals. Cyclic mice of the same age were used as controls (CTL). Exosomes were extracted from the serum of untreated cyclic mice of the same age and prepared for injection. We then performed serum exosome extraction and miRNA sequencing. Additionally, we performed liver transcriptome.