R1 Supplemental dataset.xlsx
收藏Figshare2019-02-24 更新2026-04-08 收录
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https://figshare.com/articles/R1_Supplemental_dataset_xlsx/7464818/1
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Whole transcriptome analysis of TGF-beta1 treated fibroblasts<br>Methods: RNA was purified using a miRCURY RNA isolation kit (#300110, Exiqon A/S, Vedbaek, Denmark) with an Agilent 2100 Bioanalyzer (Agilant, Santa Clara, CA, USA) used to check RNA integrity. The Illumina TrueSeq mRNA sample preparation kit (Illumina, San Diego, CA, USA) was used to prepare RNA libraries for sequencing on the Illumina HiSEq 2000. Reads were aligned using TopHat2 with the rn6 build of the rat genome. Transcript assembly and differential expression (DE) was by Cufflinks with Refseq mRNAs to guide assembly. Statistically different changes were identified by ANOVA using Partek Flow (Partek, Singapore), as previously described in https://doi.org/10.1016/j.biocel.2017.09.009.
提供机构：
TIMOTHY HEWITSON
创建时间：
2019-02-24
