Genome-wide assay of BRG1 regulated genes in inflamed colon epithelial cells

Here we focus on the function of BRG1 in colitis and colitis-associated-cancer, in the aim of finding the direct target genes of BRG1 which may response for the phenotype we observed in BRG1 conditional knock-out mice, ChIP-Seq assay is used. The cells used for ChIP-Seq is colon epithelial cells which isolated from DSS treated mice. To mimic the status of BRG1IEC KO mice, wild-type mice were treated with 2% DSS for 3 days and IECs were isolated by EDTA isolation buffer, and each sample contains pooled IECs from 5 mice. Flash frozen cells were then sent to Active Motif, Inc. for chromatin extraction and followed by immunoprecipitation using anti-BRG1 antibody (Abcam, cat # ab110641).