Files for "Discovery of natural products that modulate signaling in patient-derived cells"

FCS (Flow Cytometry Standard) files for the main text figures in the manuscript entitled "Discovery of natural products that modulate signaling in patient-derived cells". All data has quality control gating (see Supplementary Figure 2), arcsinh transformations, and compensation applied.

Figure 2 - Siderochelin MAP - Has two folders containing 28 stained and 28 fluorescent cell barcoding (FCB) files corresponding to the multiplexed activity profiling (MAP) of A. colordensis using 5 different stimuli in MV411 leukemia cells. File naming convention: RM_5_MV411_[columns of plate]_[Stained or FCB]_[number based on plate column and stained/FCB]_[well number]_Barcoded. These data files were utilized to generate the Rothko plot displayed in Figure 2c.

Figure 3 - Siderochelin MAM - Has two folders containing 30 stained and 30 FCB files corresponding to the multiplexed activity metabolomics (MAM) of A. colordensis in MV411 to identify siderochelin. File naming convention: MAM_JB_010_2_1-6_[Stained or FCB]_[003 for FCB or 004 for Stained]_[well number]_barcoded-1. These data files were utilized to generate the Rothko plot displayed in Figure 3b.

Figure 4b - Isoquinocycline B MAP - Has two folders containing 20 stained and 20 FCB files corresponding to the MAP of M. phytophila in MV411 using 5 different stimuli. File naming convention: Specimen_001_eMAMKP006_1_MV411_[FCB or Stain]_[columns of plate]_[number based on plate column and stained/FCB]_[well number]_barcoded. These data files were utilized to generate the Rothko plot displated in Figure 4b.

Figure 4c - Isoquinocycline B MAM - Has two folders containing 30 stained and 30 FCB files corresponding to the MAM of M. phytophila in MV411 to identify isoquinocycline B. File naming convention: Specimen_001_MAMJB029_MV411_[Stain or FCB]_1-6_[018 for FCB or 026 for Stained]_[well number]_barcoded. These data files were utilized to generate the Rothko plot displated in Figure 4c.

Figure 5_6 - Anthracycline CyTOF - Has 20 files corresponding to the test of three anthracyclines (isoquinocycline B (I), doxorubicin (D), and specumycin A) and vehicle (V) in five patient samples (PBMC50, AML03, AML10, AML13, and AML31).  File naming convention: c01_3_2_20_Balsamo_Human_Panel_normalized_concatenated_[Treatment: I, D, A, or V][Patient ID: 50, 03, 10, 13, or 31]_UMAP_QC3_CD45+. Has all measurements for the 35 signaling markers in the panel in addition to the axes for the UMAP displayed in the Figure 6. These data files were utilized to perform the analyses in Figures 5 and 6.