Transcriptome profiling reveals of a new cell line based model system of Amyotrophic Lateral Sclerosis model

To address the motor neuron-specific degeneration in Amyotrophic lateral sclerosis, a fatal neurodegenerative disease, we used a unique mouse stem cell differentiation system to induce cranial (NIP) and spinal motor neuron (NIL) populations. The differentiation method is published at PMID: 31157617. We monitored transcriptome changes in the two cell lines in the presence and absence of endoplasmic reticulum (ER) stress (mock and cyclopiazonic acid treatment). We used Nextseq 500 for sequencing and our data comprises expression profiles for ~13,800 transcripts. Overall design: Stem cell derieved motor neurons were subjected to protein misfolding stress. We generated transcript profiles at different time points (0hr, 6hr , 12hr) and for two replicates by deep sequencing using Illumina NextSeq500 highthroughput mode.