The selectivity of ZEL-H16 on other biogenic amine GPCRs in CRE-luciferase transcription assaysa.

aHEK-293 cells transiently expressing receptors were treated with different concentration of agonist with or without 2 µM ZEL-H16 or different concentration of ZEL-H16 separately. ZEL-H16 had no significant influence on cAMP formation alone and neither on cAMP formation induced by their corresponding agonists in the CRE- luciferase activity assays. Statistical analysis between ZEL-H16 added or not added for each concentration point was done using a t-test (PRISM software).