CarD contributes to diverse gene expression outcomes throughout the genome of Mycobacterium tuberculosis

We examined the role of the RNA polymerase binding factor CarD on gene expression throughout the Mycobacterium tuberculosis genome. We performed RNA-seq on a panel of M. tuberculosis strains with point mutations in CarD that either enhance or disrupt its ability to stabilize RNA polymerase-promoter DNA open complexes in vitro. Overall design: M. tuberculosis strains with point mutations in CarD that either enhance or disrupt its ability to stabilize RNA polymerase-promoter DNA open complexes in vitro