ZDHHC15 mediates Hippo/YAP pathway palmitoylation and regulates tumor progression [HCC1954]

The Post-translational modification of Hippo/YAP pathway members plays an important role in regulating the activation of Hippo/YAP pathway and its effects in tumors. S-Palmitoylation, also known as S-acylation, is the most common protein Lipid modification, mainly composed of palmitoyl transferases (PATs) from the DHHC (Asp His His Cys) family Catalysis. The results showed that ZDHHC15 mediated palmitoylation of KIBRA up-regulated the expression of YAP downstream target genes, and regulated the invasion function of breast cancer and ovarian cancer cells. The research results will reveal new regulatory mechanisms of the Hippo pathway Overall design: Breast cancer HCC1954 cells with stable knockdown of ZDHHC15 and shCON as a negative control. shCON and shZDHHC15 cells were extracted for RNA sequencing