BigWig Files for Dam and MSssI data

Bigwig files of the Dam and M.SssI mehtylation in S.cerevisae.We processed Dam methylated data using a snakemakeMethylFrac pipeline (https://github.com/zhuweix/snakemakeMethylFrac). This pipeline does the following: 1) The raw Illumina sequencing data (GSE230309) are aligned to the sacCer3 genome using bowtie2 v2.5.1. 2) The read fragment occupancy and 5’-ends are counted using bedtools v2.31.1. GATC half sites closer than 200 bp were filtered out because DNA fragments < 200 bp tend to be lost during sample preparation. 3) The methylated fractions for each GATC site are stored in bigwig files using pyBigWig 0.3.22 (https://github.com/deeptools/pyBigWig).Bases in the raw M.SssI nanopore sequencing data were called using dorado v0.5.0 (https://github.com/nanoporetech/dorado) with DNA model sup v3.3, 5mCG_5hmCG. The reads were aligned to sacCer3 using dorado. The methylated fraction of each CG site (5hmCG was excluded) was calculated using modkit v0.4.1 (https://github.com/nanoporetech/modkit) with parameters --cpg --combine-strands --ignore h. Methylated fractions are stored in bigwig files created using pyBigWig 0.3.22.