Chromatin accessibility of lung cancer cells reconstituted with SMARCA4 WT or SMARCA4 mutants with and without SMARCA2 depletion. Chromatin accessibility of lung cancer cells reconstituted with SMARCA4 WT or SMARCA4 mutants with and without SMARCA2 depletion

Using ATAC-seq, we identified the accessibility changes in lung cancer cells (NCI-H1944) reconstituted with SMARCA4 WT or SMARCA4 mutants. We also determined the SMARCA2-regulated accessibility program in NCI-H1944 after SMARCA2 depletion. Lastly, we identified the SMARCA2 accessibility program that is rescued by SMARCA4 WT and mutants. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. Overall design: NCI-H1944 cells were lentivirally transduced with LACZ, SMARCA4 WT and SMARCA4 mutants (K785R, E882K, T910M, R973L, R1135W, G1159V, G1162C, A1186T, R1192C, G1232S) and selected with blasticidin for 5 d. Cells were then transduced with nontargeting or SMARCA2-targeting hairpins in duplicate. After 72 h puromycin selection, cells were pelleted for ATAC-seq.