In vivo ChIP-seq analysis of Foxa1 and Foxa2 binding sites in midbrain dopaminergic progenitors and neurons. In vivo ChIP-seq analysis of Foxa1 and Foxa2 binding sites in midbrain dopaminergic progenitors and neurons

E12.5 wild-type embryos were dissected to collect ventral midbrain regions. Samples were crosslinked 10min with 1% formaldehyde and processed for chromatin extraction and Chromatin Immunoprecipitation (ChIP) following the Millipore upstate protocol. Libraries were prepared using the illumina ChIP-Seq DNA Sample Prep Kit. ChIP-Seq libraries were sequenced on the Illumina GAIIx.