Formyl peptide receptor 2 contributes to intestinal epithelium homeostasis by regulating intestinal stem cell proliferation and differentiation [scRNA-seq]

To investigate the contribution of formyl peptide receptor 2 (FPR2) to intestinal epithelium homeostasis and the underlying mechanisms, we isolated small intestinal crypts from intestinal epithelial-specific knockout (Fpr2VKO) and control (Fpr2f/f) mice, isolated epithelial cells from the crypts and performed single-cell RNA sequencing (scRNA-Seq). The transcriptome profiles of Fpr2VKO and Fpr2f/f mice were compared. Epithelial cells were isolated from small intestinal crypts of Fpr2f/f and Fpr2VKO mice and gene expression was analyzed by single-cell RNA sequencing (scRNA-seq).