Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/01/29
收藏Zenodo2020-07-29 更新2026-05-25 收录
下载链接：
https://zenodo.org/record/2600366
下载链接
链接失效反馈官方服务：
资源简介：
<strong>Project: </strong>Biophysical investigation of purified HTT protein samples
<strong>Experiment: </strong>Optimisation of HTT-HAP40 purification using heparin affinity chromatography
<strong>Date completed: </strong>2019/01/29
<strong>Rationale: </strong>The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems were conducted using heparin and FLAG affinity chromatography which showed the sample bound heparin resin – see https://zenodo.org/record/2553669. Now this need to be scaled up and tested more stringently.
提供机构：
Zenodo
创建时间：
2019-03-20
