Additive effects of environmental and demographic variation shape the repeatability of evolution across replicated experiments - R code and datasets

This repository contains all data and code necessary to reproduce the analyses and figures presented in the manuscript "Additive effects of environmental and demographic variation shape the repeatability of evolution across replicated experiments."

Included Files:

Repeatability_of_evolution_2025.RmdR Markdown script containing all analyses and figure generation used in the manuscript.

dataset_total_2025.xlsxComprehensive dataset with nematode counts from both primary and secondary treatments.

dataset_growthcurves_2025.xlsxData used to generate growth curves, serving as validation for the fitness proxy.

dataset_biosort_2025.xlsxValidation dataset used to compare manual nematode counts with those obtained via BioSorter measurements.

dataset_phyloseq_2025.xlsxRequired to generate bar plots illustrating differences in microbiota composition.

dataset_microbiota_2025.xlsxContains microbiota identified through shotgun metagenomic sequencing.

For detailed descriptions of variables and structure, please refer to the 'metadata' tab within each Excel file.