WTAP-mediated m6A modification profiling in human nasopharyngeal carcinoma cell lines

N(6)-methyladenosine (m6A) plays an important role in the tumorigenesis and progression of cancers. However, the clinical significance of m6A and their regulatory mechanisms in nasopharyngeal carcinogenesis (NPC) remain largely unknown. In this study, we used the microarray analysis to study WTAP-mediated m6A modification profiles in human nasopharyngeal carcinoma cell line, HONE-1, by comparing 3 pairs of samples with or without WTAP knockdown. RNA before and after m6A immunoprecipitation from 3 pairs of scrambled control or WTAP-silencing HONE-1 NPC cells were used to acquire different expression profiles of m6A modification and mRNA. In this way, this study provided the basis for further studying the roles and functions of WTAP-mediated m6A modification in NPC.