Single-cell transcriptomic analysis identified resistant MDSCs and a stress-tolerant gene co-expression network as common MDSC features across multiple disease settings

This experiment validated the inferred SCNV profile derived from scRNA-seq directly from DNA-level evidence using Cyto-SNP array. To provide data at the DNA-level, we cultured and harvested the samples from eight donors under two experimental conditions, control or GM-CSF + IL-6 treated PBMCs. Cytokines were added daily for three days. Then, cells were harvested and flow sorted for CD33+ cells.