Effects of different nitrogen sources on microbial community and tailing wastewater remediation

In order to dispose
of the competition problem between acidophiles and sulfate-reducing bacteria in tailing wastewater
bioremediation, research the
mechanism of different nitrogen sources to adjust microbial community, the competition experiments
of acidophiles and sulfate-reducing
bacteria were conducted. The physiochemical properties were determined
using ICP, XRD, and microbial community structure was investigated by MiSeq
sequencing. The results indicated inorganic nitrogen was positively and organic
nitrogen was negatively correlated with redox potential, dissolved sulfur and
dissolved iron (p>0.05), inorganic
nitrogen was negatively and organic nitrogen was positively correlated with pH
(p>0.05). Inorganics nitrogen
could significantly accelerate the deterioration and organic nitrogen could
promote the improvement of tailing wastewater physiochemical properties.
Inorganic nitrogen was positively correlated with Acidithiobacillus, Leptospirillum
and negatively correlated with Acidiphilium
(p>0.05). Organic nitrogen was
significantly positively correlated with Desulfosporosinus
and Desulfotomaculum (p<0.01) and negatively correlated
with Acidithiobacillus, Leptospirillum (p>0.05). Inorganic nitrogen supplementation is unfavorable and
organic nitrogen supplementation is beneficial for microbial community
adjustment to the favorable microbes.