Gene expression in primary neurons upon knockdown of Diras2
收藏Mendeley Data2024-03-27 更新2024-06-26 收录
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资源简介：
The expression of 88 target genes and three reference genes (Pgk1, Rpl13a and Sdha) was analyzed in singlets using a RealTime ready Custom Panel 384 – 96+ with Light Cycler 480 Probes Master on a LightCycler480 (Roche) using the following conditions: i) 95 °C for 10 min, ii) 45 cycles of 95 °C for 10 s, 60 °C for 30 s, 72 °C for 1 s co-terminated by a plate read. AAV2 = cells treated with AAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE AAV4 = cells treated with AAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE Scr = cells treated with AAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE Unb = untreated cells
创建时间：
2024-01-23
