2023_02_08 Cerebellum 12-39

Detection of intron 3 and intron 12 retaining MAPT species by digital droplet PCR in cerebellum samples of non-demented individuals and Alzheimer's disease patients. Each retention was detected in a different fluorophore. There are species that retain both introns TIR³⁺¹²-MAPT (Fam +, Hex +) which would be translated into DW-Tau (double intron retention), species that retain only intron 3 TIR³-MAPT (Fam +, Hex -) which would be translated into NW-Tau (Nt truncation) or species that retain only intron 12 TIR¹²-MAPT (Fam -, Hex +) which would be translated into CW-Tau (Ct truncation).