INVESTIGATION OF TICK-BORNE PATHOGENS RESISTANCE MARKERS USING NEXT GENERATION SEQUENCING

BACTERIAL COMMUNITIES AND THEIR FUNCTIONAL ANTIBIOTIC RESISTANT MARKERS IN AMBLYOMMA AND HYALOMMA TICKS.The study aims to determine the genetic composition of tick-borne bacterial communities and investigating their resistance biomarkers using next-generation sequencing.THE SPECIFIC OBJECTIVES:To identify and determine tick-borne bacterial communities associated with ticks parasitizing Nguni cattle breeds from Roodeplaat ARC-research farm.To apply 16S rRNA metagenomics approach to characterize bacterial communities associated with Amblyomma and Hyalomma tick species.To investigate functional antimicrobial resistance biomarkers using bioinformatics (tool: PICRUSt).