Additional file 2: of Stability of gene expression by primary bronchial epithelial cells over increasing passage number

Figure S2. Comparison of gene expression between asthmatic and healthy BECs at passage 1 (P1). Expression of genes related to airway remodeling (panel A.; TGFβ1, TGFβ2, MUC5AC, activin A, and FSTL3), innate immunity and immunomodulatory genes (panel B.; CXCL10, IFIH1, IL-33, and TSLP), and expression of genes associated with airway epithelial basal cells, ciliogenesis, and epithelial tight junctions (panel C.; TP63, KRT5, TUBB4A, TJP1, OCLN, and FOXJ1) by primary asthmatic (grey plots) and healthy (white plots) BECs at P1 (n = 6 asthma donors, n = 6 healthy donors). Expression of each gene (normalized to GAPDH) relative to the median of healthy BECs are presented as box-and-whisker plots which depict the interquartile range and median (the ends of each box represent the upper and lower quartiles, error bars represent the maximum and minimum, and the horizontal line within the box represents the median). The Wilcoxon signed rank test was used to test differences in expression of specific genes between asthmatic and healthy BECs. (EMF 109 kb)